Alginate lyase

Alginate lyase is produced by our company through liquid deep fermentation and refined extraction from excellent strains introduced from abroad. It can effectively hydrolyze alginate under neutral pH 6-8 conditions and can be widely used in the processing of brown algae, sodium alginate, and alginate oligosaccharides.

I. Principle:

Alginate is a linear polysaccharide composed of α-L-guluronic acid and its isomer β-D-mannuronic acid linked by 1,4 glycosidic bonds. Alginate lyase degrades alginate through β-elimination catalysis, forming an unsaturated double bond between C4 and C5, and producing a degradation process at the reducing end, ultimately degrading the long-chain polysaccharide into short-chain oligosaccharides.

II. Product Specifications:

Liquid type: Brownish-yellow liquid 10000U/ml 25KG/barrel

Solid type: Yellow powder 250000U/ml 25KG/barrel

No less than the calibrated enzyme activity at room temperature for six months, avoid high temperature and sun exposure.

Enzyme activity definition: 1 ml of liquid enzyme degrades alginate to produce 1 μg of reducing sugar per minute at 40 degrees, which is 1 enzyme activity unit, expressed as U/ml;

III. Usage:

In the production of pure alginate degradation, taking solid enzyme as an example (the amount of liquid enzyme added is generally 5~7.5%; the amount of solid enzyme added is generally 0.2~0.3%), 2% sodium alginate aqueous solution (sodium alginate: Solarbio, reagent grade), initial viscosity of about 700, under the condition of stirring at 35 degrees, add 0.3% of solid enzyme (based on sodium alginate), after 5 minutes, the viscosity drops to 10, after 1 hour, the hydrolysis is completed, the alginate solution becomes a watery liquid, the product molecular weight is 200~2000, and the degree of polymerization is 2~10.

IV. Difference between enzymatic method and traditional method:

Our company will work with you to improve the process and solve problems. Please let us know your needs, and we will do our best to serve you.